ABSTRACT
The localisation and enzymatic activity of aryl sulphatase and non-specific esterase in normal colon, colon adenocarcinoma, 5-fluorouracil and CCNU treated cells were studied respectively. Intracytoplasmic granular reactions were observed for all tissues. The enzymatic activity was found to be more intense in malignant cells of colon. The increased activities of these enzymes in treated cells might be considered as an index of the in creased fragility of lysosomes and degeneration of some tumour cells which acompanied by a decrease in T-Cells function
Subject(s)
Animals, Laboratory , Adenocarcinoma , Fluorouracil , Lomustine , Histocytochemistry , Mice , Models, Animal , Fluorouracil/immunology , Lomustine/immunology , Arylsulfatases , CarboxylesteraseABSTRACT
Deoxyribonucleic acid [DNA] and glucose 6- phosphate dehydrcgenase [G6PD] enzyme were studied in human normal lymph node and Hodgk in's disease cells. It has been observed that DNA was present in chromatin materials, whereas [G6P D] was present as granules distributed uniformaly in the cytoplasm. Under the effect of endoxan, a decreased activity of DNA and G6PD has been noticed. This could be explained on the basis that alkylation of phosphate groups was the main point of attack on the DNA which result in the breakdown of the DNA molecule
Subject(s)
Humans , Cyclophosphamide , Cyclophosphamide/adverse effects , DNA Damage , Glucose-6-Phosphate , Lymph Nodes , Histocytochemistry/methodsABSTRACT
The localization and concentration of succinic dehydrogenase [SDH] and lactic dehydrogenase [LDH] in colon adenocarcinoma before and after the application of 5-Fluorouracil [5-Fu] and 1- [2-Chloroethyl] 3-CylohexanylM-Nitrosourea [CCNU] drugs were studied. It was found that there was a marked decrease in the concentration of lactic dehydrogenase [LDH] in the treated cells. On the contrary, the concentration of succinic dehydrogenase [SDH] was found to be increased